Anti-Aanat Antibody Picoband™

Product Name

Anti-Aanat Antibody Picoband™

View all Serotonin N-acetyltransferase Antibodies

SKU/Catalog Number

A03784-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aanat Antibody Picoband™ catalog # A03784-1. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Aanat Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03784-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Aanat recombinant protein (Position: C24-C205).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03784-1 is reactive to AANAT in Mouse

Applications

A03784-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

23.344kDa

Background of Serotonin N-acetyltransferase

Aralkylamine N-acetyltransferase (AANAT) (EC 2.3.1.87), also known as arylalkylamine N-acetyltransferase or serotonin N-acetyltransferase (SNAT), is an enzyme that is involved in the day/night rhythmic production of melatonin, by modification of serotonin. It is in humans encoded by the ~2.5 kb AANAT gene containing four exons, located on chromosome 17q25. The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Aanat using anti-Aanat antibody (A03784-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aanat antigen affinity purified polyclonal antibody (Catalog # A03784-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aanat at approximately 23 kDa. The expected band size for Aanat is at 23 kDa.

Gene/Protein Information For AANAT (Source: Uniprot.org, NCBI)

Alternative Names

AA-NAT; aralkylamine N-acetyltransferaseEC 2.3.1.87; arylalkylamine N-acetyltransferase; Serotonin acetylase; SNATserotonin N-acetyltransferase AANAT DSPS, SNAT aralkylamine N-acetyltransferase serotonin N-acetyltransferase|arylalkylamine N-acetyltransferase|serotonin acetylase

For more info on AANAT, check out the AANAT Infographic

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In this infographic, you will see the following information for AANAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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